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Ran nuclear/cytoplasmic ratio The intensity of staining for Ran in the cytoplasm and nucleus following transfection of HeLa cells expressing GFP-tubulin or MCF or PC3 cells with non-silencing siRNA or siRNA to PlexinB1 was measured using ImageJ (NIH) using DAPI staining to outline the nucleus and tubulin or Plexin staining to identify the cytoplasm
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