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Differentiation of 3T3-L1 pre-adipocytes 3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work.[18] Briefly, standard culture media was supplemented with scheduled addition of adipogenic agents [3-isobutyl-1methyl xanthine (IBMX), Sigma Aldrich
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Size:200 mg
In that setting, patients received high-dose NAD+ daily for about 4-10 days to alleviate acute withdrawal symptoms, followed by tapering to less frequent booster injections over the ensuing weeks
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