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Key Takeaways Lyme disease blood tests detect antibodies to Borrelia burgdorferi , not the bacterium itself — timing of testing is critical The UK standard is a two-tier approach: ELISA screening followed by immunoblot confirmation if positive or equivocal Testing within the first two to four weeks of infection frequently produces false negative results as antibodies haven’t yet developed A bull’s-eye rash (erythema migrans) is sufficient for clinical diagnosis — a blood test is not always required in early infection Lyme disease is increasingly common in Scotland, particularly in rural and wooded areas with high tick populations Private testing offers faster access to results for patients who have been exposed to ticks and are experiencing symptoms What is Lyme Disease and How is it Transmitted