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Dynamic modeling of Nrf2 pathway activation in liver cells after toxicant exposure | Scientific Reports

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Each sample was then split into two fraction (100 μL) and treated as follows: For H 2 S measurement, 100 μL of each sample was precipitated with 1 mL of absolute MeOH at –40 °C for 2 h, centrifuged (16,000 × g at 0 °C), and obtained supernatant was evaporated and the residue was resuspended and sonicated in 100 μL of 50 mM HEPES buffer (pH 7.4)

Assay Glutathione Peroxidase Activity in cell/tissue extracts with Glutathione Peroxidase Activity Assay Kit (ab219926)