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In reality, “safe” depends on the specific contaminant, its concentration, the duration of exposure, and whether sensitive groups such as infants or pregnant people are affected.That assumption breaks when “tested” refers to a public water system report, a one-time home kit, or a lab result taken from the wrong faucet at the wrong time

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Western blot analysis The kidney organoids were homogenized in boiling lysis buffer (1% SDS, 1 mM sodium orthovanadate, and 10 mM Tris, pH 7.4), and the protein concentration was determined with a BCA Protein Assay Kit (Pierce Biotechnology Inc., Rockford, IL, USA)