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Single-cell metabolic flux analysis (scFEA) in colorectal cancer further revealed that it is the Glu-to-GSH metabolic step, downstream of GLS, rather than Glu-to-α-KG flux, that predominantly governs T cell cytotoxicity, pharmacological inhibition of GCLC elevated immunoproteasome activity, enhanced MHC-I-mediated tumor antigen presentation, and sensitized colorectal tumors to anti-PD-1 therapy [193]
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