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As mentioned earlier, a major role of NADPH is to detoxify ROS, which are intracellular chemical species that contain oxygen (O 2 ) and are reactive toward lipids, proteins, and DNA

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547 Various laboratory techniques, such as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), allele-specific oligonucleotide polymerase chain reaction (ASO-PCR), single-strand conformation polymorphism (SSCP), long-range PCR, and Southern blot, have traditionally been used to screen for point mutations and deletions in mtDNA