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To investigate whether one or both putative binding sites were functional, we cloned fragments of 98 and 96 bp of the human IGF-1 3′ UTR mRNA (NM_ 000618) containing the two putative miRNA-binding sites into the psiCHEK-2 vector (Supplementary Fig
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To test the effects of GHK and GHK-PEG on cytotoxicity in SH-SY5Y cells, cells were plated in 96 well plates at 5000 cells per well and incubated for 24 hrs
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