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Perspective Chapter: Iron Chelation Inhibits Reduced Glutathione (GSH) as a Prooxidant in Iron-Mediated Hemoglobin Oxidation in Purified Hemoglobin | IntechOpen

In this work, five genes ( ldhD, dpoIII, dnaG, gyrA , and gyrB ) were evaluated as internal controls for RT-qPCR, using the primers described in Table 1 (Desroche et al., −ΔΔCT method, and the amount of target RNA was adjusted to the geometric mean of the five internal controls as previously described (Livak and Schmittgen, Table 1 Gene descriptions and the corresponding primer sequences used for validation of RNA-seq results by RT-qPCR

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Cells were then stained with the ROS-sensitive probe dihydroethidium (DHE) for 1 h and their fluorescence levels were measured by flow cytometry