glutathione peroxidase estimation Enhanced protocol for measuring activity using a new glutathione peroxidase-Tiron assay Sigma aldrich

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Oxidized and reduced (dihydrolipoic acid, DHLA) forms of ALA have been found to chelate heavy metals (Ou et al., 1995), scavenge various reactive oxygen species (ROS) (Packer et al., 1995), and affect antioxidant gene expression (Shay et al., 2009)

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limiting factor of the coupled reactions.