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His-tagged proteins are purified using immobilized metal affinity chromatography (IMAC) resins, which come in many flavors of metal cores—the most popular being nickel- (Ni 2+ ) or cobalt-based (Co 2+ ) resins
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However, following ingestion of a large overdose (150 mg/kg or greater) the glucuronide and sulfate conjugation pathways are saturated resulting in a larger fraction of the drug being metabolized via the P-450 pathway
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