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During the degranulation and release, perforin polymerizes and forms pores in the target cell membrane to facilitate granzymes delivery into target cells, inducing apoptosis through different pathways, including caspases-3/7 activation, cytochrome C release from mitochondria driven by Bak/Bax, and IL-1β regulation by caspase-1 [32]
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This single change sterically hinders DPP-4, rendering the analogue essentially resistant to proteolysis and preserving its biological integrity for many hours after administration [29,32]
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