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2 mM riboflavin (0.1 mL) was added in 3 mL of reaction mixture (13.33 mM methionine, 75 µM NBT, 0.1 mM EDTA, 50 mM phosphate buffer (pH 7.8), 50 mM sodium carbonate, 0.1 mL enzyme extract) and placing the tubes under two 15 W fluorescent lamps for 15 min to start the reaction

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