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The labeling reaction was quenched with the addition of urea dye [7 M urea, 1 mM EDTA, 0.05% (w/v) xylene cyanol, 0.05% (w/v) bromophenol blue, 10% (v/v) phenol], loaded onto a denaturing 8% (w/v) polyacrylamide/7 M urea/1X Tris-Borate-EDTA (TBE) gel, and electrophoresed in 1X TBE buffer for 75 min
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