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When Glutamate Detection Reagent, which contains glutamate dehydrogenase (GlutDH), NAD+, Reductase, Reductase Substrate and Luciferase, is added to a sample containing glutamate at a 1:1 ratio, the enzyme-coupled reactions start and run simultaneously
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For the model of cultured rat lens ex vivo, the 12 lenses of 6-week rats were isolated and cultured in serum free M199 media media containing PBS (sham), 10 μM DEX or 10 μM DEX + 20 μM RU486 (antagonist of GR) for three days
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